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Natural infection by mastreviruses was investigated in chickpea (Cicer arietinum) and other dicotyledonous crops and weeds in grain manufacturing areas of Queensland and northern New South Wales, Australia, from 2000 to 2005. Altogether, 33 639 plants comprising 31 species and 10 dicot households have been screened for infection by a tissue-blot immunoassay that did not distinguish between mastrevirus strains or species. Nine plant species in three families had been identified as natural hosts. Chickpea was infected all through the region although infection incidence didn’t exceed 5%. Infection was rare in faba bean (Vicia faba), canola (Brassica napus), and mustard (B. Individual mastreviruses have been recognized by comparing their IC-PCR amplicons by a combination of methods. Among forty two isolates from 41 chickpea plants, one was typical Tobacco yellow dwarf virus (TYDV) and the others had been three lately distinguished strains together with two proposed novel species: 34 Chickpea chlorosis virus strain A, six Chickpea chlorosis virus pressure B, and one Chickpea redleaf virus. All of 10 isolates from 10 turnip Weed strains plants had been TYDV-B, a pressure distinct from typical TYDV. The symptoms associated with mastrevirus infection in chickpea included foliar chlorosis or reddening, stunting, and often phloem browning. The potential for losses in winter and summer time grown subject crops is mentioned.